| Registro completo |
| Provedor de dados: |
BJID
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| País: |
Brazil
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| Título: |
Signal to cut-off (S/CO) ratio and detection of HCV genotype 1 by real-time PCR one-step method: is there any direct relationship?
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| Autores: |
Albertoni,Guilherme
Arnoni,CP
Araújo,PRB
Carvalho,FO
Barreto,JA
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| Data: |
2010-04-01
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| Ano: |
2010
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| Palavras-chave: |
HCV
HCV genotype 1
Real-time PCR
ELISA
RNA extraction
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| Resumo: |
BACKGROUND: Polymerase chain reaction (PCR) methods play an essential role in providing data related to diagnosis, monitoring and treatment of hepatitis C virus (HCV) infection. EIA results are reported as ''reactive'' or ''non reactive'' and EIA S/CO ratio may also be reported as ''high'' or ''low.'' This study aimed to evaluate the performance of a real-time RT-PCR and assess whether there is relationship between S/CO and PCR results. STUDY DESIGN AND METHODS: Sera from blood donors were analyzed by Enzyme-Linked Immunosorbent Assay (ELISA) and RT-PCR assay to detect HCV infection. RESULTS: The RT-PCR assay to genotypes 1a/b showed an acceptable linear response in serial dilutions. The samples were divided into two groups based on their serological results: group A - S/CO ratio < 3 (60 samples) and group B - S/CO ratio > 3 (41 samples). Viral loads were confirmed positive in group B samples in 90%, and in group A samples were confirmed positive in only 13% by RT-PCR. CONCLUSION: The methodology used was able to detect the presence of RNA-HCV genotype I in 90% of the samples serologically positive in group B. All negative samples were sent to search for other genotypes of HCV (genotypes 2-6) and were confirmed as negative. These data suggests that these negative samples may have HCV RNA viral load below the detection limit of our test (310 IU/ mL), or a false positive result in serological test, or spontaneous viral clearance occurred.
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702010000200006
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| Editor: |
Brazilian Society of Infectious Diseases
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| Relação: |
10.1590/S1413-86702010000200006
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| Formato: |
text/html
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| Fonte: |
Brazilian Journal of Infectious Diseases v.14 n.2 2010
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| Direitos: |
info:eu-repo/semantics/openAccess
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